Composite

Part:BBa_M34543:Design

Designed by: Divya Arcot   Group: MIT BE 20.20 - S11   (2011-05-09)


Exported Yeast TIMP-1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2027
    Illegal PstI site found at 2056
    Illegal PstI site found at 2177
    Illegal PstI site found at 2455
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2027
    Illegal PstI site found at 2056
    Illegal PstI site found at 2177
    Illegal PstI site found at 2455
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2675
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2027
    Illegal PstI site found at 2056
    Illegal PstI site found at 2177
    Illegal PstI site found at 2455
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2027
    Illegal PstI site found at 2056
    Illegal PstI site found at 2177
    Illegal PstI site found at 2455
    Illegal NgoMIV site found at 224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 874


Design Notes

We wanted to use a signal sequence which exported the greatest amount of the TIMP-1 protein, so we chose the alpha F sequence over others. We also chose to use yeast as a chassis because TIMP-1 is a human gene and yeast would be able to glycosylate it. However, yeast glycosylate the protein in a manner that is slightly different than in humans.


Source

The human TIMP-1 gene is located on the X chromosome. For the terminator sequence: pOBD2. Promoter is from the Parts Registry (Part BBa_I766556) Signal Sequence Source: http://peds.oxfordjournals.org/content/11/2/75.full.pdf (use alpha F sequence)

References